Not Medical/Legal Advice: Yes, it will. Just like regular tobacco, hookah contains nicotine.
When one smokes, nicotine inhaled is then metabolized into cotinine in organs such as the lungs and the liver, and subsequently excreted in the urine. Aside from nicotine, cotinine is a reliable biomarker and indicator for nicotine use, detectable in the blood, urine, and saliva.
The length of time that nicotine stays in the body and cotinine can be a reliable indicator of nicotine use depends on the amount of tobacco smoked, the number of years smoking tobacco, and the type of nicotine test performed. It can be as short as 2 days, or as long as 3 months.
An estimated range of times during which nicotine can be detected by various testing methods:
Urine Test: 3-4 days
Blood Test: 1-3 days
Saliva Test: 2-4 days
Like many other drugs, nicotine can be detected with a hair follicle drug test for up to 90 days.
Find out the negative health effects of hookah at the Huffington Post.
Tip! Tobacco cravings can wear you down when you're trying to quit smoking or chewing tobacco. Here are 10 ways to resist tobacco cravings shared by Mayoclinic.org.